Beilstein J. Org. Chem.2017,13, 1498–1506, doi:10.3762/bjoc.13.148
demonstrate the importance of the right choice of the membrane protein stabilizer for biohybridcatalysis.
Results and Discussion
For solubilizing the transmembrane protein FhuA ΔCVFtev PE–PEG and MPD were applied as stabilizing agent and phenylacetylene polymerization was performed as model reaction (Figure
precipitation leading to increased yields.
The membrane protein FhuA is robust towards external influences such as increased temperatures and pH values. The catalyst and substrate scope in biohybridcatalysis can be fine-tuned choosing a suitable stabilizing agent as shown in this report. These results may
inspire the tailoring of membrane proteins as catalysts in the field of biohybridcatalysis.
Experimental
General comments
All used chemicals used were of analytical grade or higher quality, purchased from Applichem (Darmstadt, Germany) or Sigma-Aldrich Chemie (Taufkirchen, Germany).
All operations were
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Graphical Abstract
Figure 1:
Phenylacetylene polymerization of FhuA ΔCVFtev [29] refolded in a polymer or small amphiphilic molecule...
Beilstein J. Org. Chem.2016,12, 1314–1321, doi:10.3762/bjoc.12.124
: artificial Diels-Alderase; biohybridcatalysis; copper enyzme; membrane protein; Introduction
So-called artificial metalloenzymes have attracted attention over the last decade [1][2][3][4][5][6][7][8][9]. Incorporation of an organometallic cofactor into proteins offers new possibilities to expand the
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Graphical Abstract
Scheme 1:
Syntheses to Cu(I) complex bearing a NHC ligand.